KMID : 1144820190250040398
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´ëÇÑÀÇ»ý¸í°úÇÐȸÁö 2019 Volume.25 No. 4 p.398 ~ p.406
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Development of Genetically Modified Tumor Cell Containing Co-stimulatory Molecule
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Kim Hong-Sung
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Abstract
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Cancer immunotherapy using gene-modified tumor cells is safe and customized cancer treatment method. In this study, we made gene-modified tumor cells by transferring costimulatory molecules, 4-1BBL and OX40L, into tumor cells using lentivirus vector, and identified anti-cancer effect of gene-modified tumor cells in CT26 mouse colorectal tumor model. We construct pLVX-puro-4-1BBL, -OX40L vector for lentivirus production and optimized the transfection efficiency and transduction efficiency. The transfection efficiency is maximal at DNA:cationic polymer = 1:0.5 and DNA 2 ¥ìg for lentivirus production. Then, the lentiviral including 4-1BBL and OX40L was used to deliver CT26 mouse tumor cells to establish optimal delivery conditions according to the amount of virus. The transduction efficiency is maximal at 500 ¥ìL volume of lentiviral stock without change in cell shape or growth rate. CT26-4-1BBL, CT26-OX40L significantly inhibited the tumor growth compare with CT26-WT or CT26-¥â-gal cell line. These data showed the possibility the use of genetically modified tumor cells with costimulatory molecule as cancer immunotherapy agent.
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KEYWORD
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Genetically modified tumor cell, Gene transfection, Transfection efficiency, Tumor growth pattern
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